A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation.
Identifieur interne : 002476 ( Main/Exploration ); précédent : 002475; suivant : 002477A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation.
Auteurs : Elizabeth A. Pharo [Australie] ; Kylie N. Cane [Australie] ; Julia Mccoey [Australie] ; Ashley M. Buckle [Australie] ; W H Oosthuizen [Afrique du Sud] ; Christophe Guinet [France] ; John P Y. Arnould [Australie]Source :
- Gene [ 1879-0038 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Colostrum (enzymologie), Expression des gènes, Femelle, Glandes mammaires animales (métabolisme), Grossesse, Inhibiteurs trypsiques (), Inhibiteurs trypsiques (métabolisme), Lactation (métabolisme), Mammifères (métabolisme), Otaries à fourrure (génétique), Otaries à fourrure (métabolisme), Similitude structurale de protéines, Simulation numérique, Suidae, Trypsine (métabolisme).
- MESH :
- enzymologie : Colostrum.
- génétique : Otaries à fourrure.
- métabolisme : Glandes mammaires animales, Inhibiteurs trypsiques, Lactation, Mammifères, Otaries à fourrure, Trypsine.
- Animaux, Bovins, Expression des gènes, Femelle, Grossesse, Inhibiteurs trypsiques, Similitude structurale de protéines, Simulation numérique, Suidae.
English descriptors
- KwdEn :
- Animals, Cattle, Colostrum (enzymology), Computer Simulation, Female, Fur Seals (genetics), Fur Seals (metabolism), Gene Expression, Lactation (metabolism), Mammals (metabolism), Mammary Glands, Animal (metabolism), Pregnancy, Structural Homology, Protein, Swine, Trypsin (metabolism), Trypsin Inhibitors (chemistry), Trypsin Inhibitors (metabolism).
- MESH :
- chemical , chemistry : Trypsin Inhibitors.
- chemical , metabolism : Trypsin, Trypsin Inhibitors.
- enzymology : Colostrum.
- genetics : Fur Seals.
- metabolism : Fur Seals, Lactation, Mammals, Mammary Glands, Animal.
- Animals, Cattle, Computer Simulation, Female, Gene Expression, Pregnancy, Structural Homology, Protein, Swine.
Abstract
The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk.
DOI: 10.1016/j.gene.2015.11.042
PubMed: 26639991
Affiliations:
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Pharo</name><affiliation wicri:level="4"><nlm:affiliation>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia. Electronic address: e.pharo@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Cane, Kylie N" sort="Cane, Kylie N" uniqKey="Cane K" first="Kylie N" last="Cane">Kylie N. Cane</name><affiliation wicri:level="4"><nlm:affiliation>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia. Electronic address: kylie.cane@gmail.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Mccoey, Julia" sort="Mccoey, Julia" uniqKey="Mccoey J" first="Julia" last="Mccoey">Julia Mccoey</name><affiliation wicri:level="1"><nlm:affiliation>Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia. Electronic address: julia.mccoey@gmail.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800</wicri:regionArea><wicri:noRegion>VIC 3800</wicri:noRegion></affiliation></author><author><name sortKey="Buckle, Ashley M" sort="Buckle, Ashley M" uniqKey="Buckle A" first="Ashley M" last="Buckle">Ashley M. Buckle</name><affiliation wicri:level="1"><nlm:affiliation>Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia. Electronic address: ashley.buckle@monash.edu.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800</wicri:regionArea><wicri:noRegion>VIC 3800</wicri:noRegion></affiliation></author><author><name sortKey="Oosthuizen, W H" sort="Oosthuizen, W H" uniqKey="Oosthuizen W" first="W H" last="Oosthuizen">W H Oosthuizen</name><affiliation wicri:level="1"><nlm:affiliation>Oceans and Coasts, Department of Environmental Affairs, Private Bag X2, Roggebaai 8012, South Africa. Electronic address: oosthuiz@environment.gov.za.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Oceans and Coasts, Department of Environmental Affairs, Private Bag X2, Roggebaai 8012</wicri:regionArea><wicri:noRegion>Roggebaai 8012</wicri:noRegion></affiliation></author><author><name sortKey="Guinet, Christophe" sort="Guinet, Christophe" uniqKey="Guinet C" first="Christophe" last="Guinet">Christophe Guinet</name><affiliation wicri:level="1"><nlm:affiliation>Centre d'Etudes Biologiques de Chizé, CNRS, 79360 Villiers en Bois, France. Electronic address: guinet@cebc.cnrs.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre d'Etudes Biologiques de Chizé, CNRS, 79360 Villiers en Bois</wicri:regionArea><wicri:noRegion>79360 Villiers en Bois</wicri:noRegion><wicri:noRegion>79360 Villiers en Bois</wicri:noRegion></affiliation></author><author><name sortKey="Arnould, John P Y" sort="Arnould, John P Y" uniqKey="Arnould J" first="John P Y" last="Arnould">John P Y. Arnould</name><affiliation wicri:level="4"><nlm:affiliation>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia; School of Life and Environmental Sciences, Deakin University, Burwood, VIC 3125, Australia. Electronic address: john.arnould@deakin.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia; School of Life and Environmental Sciences, Deakin University, Burwood, VIC 3125</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Colostrum (enzymology)</term><term>Computer Simulation</term><term>Female</term><term>Fur Seals (genetics)</term><term>Fur Seals (metabolism)</term><term>Gene Expression</term><term>Lactation (metabolism)</term><term>Mammals (metabolism)</term><term>Mammary Glands, Animal (metabolism)</term><term>Pregnancy</term><term>Structural Homology, Protein</term><term>Swine</term><term>Trypsin (metabolism)</term><term>Trypsin Inhibitors (chemistry)</term><term>Trypsin Inhibitors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Colostrum (enzymologie)</term><term>Expression des gènes</term><term>Femelle</term><term>Glandes mammaires animales (métabolisme)</term><term>Grossesse</term><term>Inhibiteurs trypsiques ()</term><term>Inhibiteurs trypsiques (métabolisme)</term><term>Lactation (métabolisme)</term><term>Mammifères (métabolisme)</term><term>Otaries à fourrure (génétique)</term><term>Otaries à fourrure (métabolisme)</term><term>Similitude structurale de protéines</term><term>Simulation numérique</term><term>Suidae</term><term>Trypsine (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Trypsin Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Trypsin</term><term>Trypsin Inhibitors</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Colostrum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Colostrum</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fur Seals</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Otaries à fourrure</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fur Seals</term><term>Lactation</term><term>Mammals</term><term>Mammary Glands, Animal</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glandes mammaires animales</term><term>Inhibiteurs trypsiques</term><term>Lactation</term><term>Mammifères</term><term>Otaries à fourrure</term><term>Trypsine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Computer Simulation</term><term>Female</term><term>Gene Expression</term><term>Pregnancy</term><term>Structural Homology, Protein</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Expression des gènes</term><term>Femelle</term><term>Grossesse</term><term>Inhibiteurs trypsiques</term><term>Similitude structurale de protéines</term><term>Simulation numérique</term><term>Suidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk.</div></front></TEI><affiliations><list><country><li>Afrique du Sud</li><li>Australie</li><li>France</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Pharo, Elizabeth A" sort="Pharo, Elizabeth A" uniqKey="Pharo E" first="Elizabeth A" last="Pharo">Elizabeth A. Pharo</name></region><name sortKey="Arnould, John P Y" sort="Arnould, John P Y" uniqKey="Arnould J" first="John P Y" last="Arnould">John P Y. Arnould</name><name sortKey="Buckle, Ashley M" sort="Buckle, Ashley M" uniqKey="Buckle A" first="Ashley M" last="Buckle">Ashley M. Buckle</name><name sortKey="Cane, Kylie N" sort="Cane, Kylie N" uniqKey="Cane K" first="Kylie N" last="Cane">Kylie N. Cane</name><name sortKey="Mccoey, Julia" sort="Mccoey, Julia" uniqKey="Mccoey J" first="Julia" last="Mccoey">Julia Mccoey</name></country><country name="Afrique du Sud"><noRegion><name sortKey="Oosthuizen, W H" sort="Oosthuizen, W H" uniqKey="Oosthuizen W" first="W H" last="Oosthuizen">W H Oosthuizen</name></noRegion></country><country name="France"><noRegion><name sortKey="Guinet, Christophe" sort="Guinet, Christophe" uniqKey="Guinet C" first="Christophe" last="Guinet">Christophe Guinet</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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